MORPHOLOGICAL CHARACTER AND MOLECULAR IDENTIFICATION WITH COI GENE MARKER OF HORSESHOE CRABS (Tachypleus gigas) AT COASTAL WATERS OF NORTHERN JAVA ISLAND
The existence of horseshoe crabs Tachypleus gigas is currently on the threatened condition and its population status is also unclear. The objectives of this research were to asses the horseshoe crabs morphological characters and to identify based on molecular marker of Cytochrome Oxydase Subunit I (COI) gene. The samples were collected from five locations (Segara Menyan Subang, Ujung Kulon Banten, Tambak Lorok Semarang, Betah Walang Demak, and Kenjeran Surabaya) in October 2014 until June 2015. A total of 27 parameters for 62 individuals were used to determine connectivity and character identifier among populations. Data were tested using non-parametric analysis. While, the 5 individuals from each sites were used for molecular analysis including several stages of the DNA extraction, DNA electrophoresis, amplification and visualization of DNA fragments, and DNA sequencing. The results obtained based on morphological characters that T. gigas of Surabaya formed a unique group due to its body size (X1-X2 and X4-X16) was larger than the other 5 body parts (X22, X23, X26-X28) of Semarang, Demak, Subang, and Banten. According to molecular results, there were 650 bp conserve nucleotide sequences. Analysis using software MEGA 5.0 showed that there were 73 existence of specific nucleotide sites that can be used to distinguishes between T. gigas in-groups and out-groups (T. gigas from Central Arabian Sea, T. tridentatus from Sweden, and Carcinoscorpius rotundicauda from USA).
Keywords: horseshoe crabs, Java coast, morphology, identification molecular, CO1 gene.
The author submitting the manuscript must understand and agree that the copyright of the article manuscript must be submitted/transferred to the Jurnal Ilmu dan Teknologi Kelautan Tropis. This work is licensed under the Creative Commons Attribution-ShareAlike 4.0 (CC BY-SA) International License in which the Author and Reader can copy and redistribute the material in any media or format, and remix, modify and build material for any purpose, but they must provide appropriate credit (citing articles or content), provide a link to the license, and indicate whether there is a change. If you mix, change, or create material, you must distribute your contribution under the same license as the original.