Konservasi In Vitro Pamelo (Citrus maxima (Burm.) Merr.) melalui Pertumbuhan Lambat

  • Kartika Ning Tyas Pusat Konservasi Tumbuhan Kebun Raya Bogor-LIPI, Jl. Ir. H. Juanda 13, Bogor 16122, Indonesia 2Departemen Agronomi dan Hortikultura, Fakultas Pertanian, Institut Pertanian Bogor
  • Slamet Susanto Departemen Agronomi dan Hortikultura, Fakultas Pertanian, Institut Pertanian Bogor (Bogor Agricultural University), Jl. Meranti, Kampus IPB Darmaga, Bogor 16680, Indonesia
  • Iswari Saraswati Dewi Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber daya Genetik Pertanian Jl. Tentara Pelajar No 3A, Cimanggu, Bogor 16111, Indonesia
  • Nurul Khumaida Departemen Agronomi dan Hortikultura, Fakultas Pertanian, Institut Pertanian Bogor (Bogor Agricultural University), Jl. Meranti, Kampus IPB Darmaga, Bogor 16680, Indonesia

Abstract

Indonesia is one of the countries which have abundant germplasm of pummelo (Citrus maxima (Burm.) Merr.). The
pummelo germplasm must be conserved to prevent its extinction due to biotic and abiotic stresses. In vitro conservation
using slow growth technique can be considered as an alternative of ex-situ conservation. Two experiments were conducted
to obtain suitable medium to conserve pummelo in vitro. The first experiment was conservation using modified concentration
of MS and sucrose. MS medium concentrations were 1/2MS and MS, while sucrose concentrations were 0, 1, 2 and 3%. The
second experiment was conservation using osmoticum and retardant in MS medium. There were six combinations of MS
medium supplemented with osmoticum and retardant, i.e. MS + sucrose 3%, MS + sucrose 3% + paclobutrazol 7.5 ppm, MS
+ sucrose 3% + paclobutrazol 15 ppm, MS + sorbitol 2%, MS + sorbitol 2% + paclobutrazol 7.5 ppm, MS + sorbitol 2% +
paclobutrazol 15 ppm. The results showed that reducing MS medium and sucrose consentration decreased leaf number and
shoot length but increased root number and length. The combination of osmoticum and retardant reduced shoot length, leaf number, root number and length. Based on the planlet visual and inhibition of growth through the decrease of leaf number, shoot and root length, the best medium to preserve pummelo was MS + sorbitol 2% + paclobutrazol 7.5 ppm.
Keywords: Citrus maxima, osmoticum, preservation, retardant, slow growth
Published
2013-10-06
How to Cite
Tyas, K. N., Susanto, S., Dewi, I. S., & Khumaida, N. (2013). Konservasi In Vitro Pamelo (Citrus maxima (Burm.) Merr.) melalui Pertumbuhan Lambat. Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy), 41(1). https://doi.org/10.24831/jai.v41i1.7073