Pengaruh L-Sistein terhadap Efisiensi Transformasi Genetik Jagung (Zea mays) Menggunakan Agrobacterium

  • Setyo Dwi Utomo


An efficient procedure of genetic transformation ultimately can accelerate the process of cultivar development of maize.  The objective of this study was to evaluate the effect of L-cysteine added to co-cultivation medium on the efficiency of Agrobacterium-mediated transformation of two genotypes of maize.  Explants of immature embryos were isolated from immature ears genotypes Hi-II and Tom Thumb harvested 11-13 days after pollination.  Then explants were inoculated with Agrobacterium strain C58C1 carrying pPTN345 vector and cultured in co-cultivation medium for 2 days then on delay medium for 14 days, on selection medium for 4 x 14 days, on regeneration medium, and finally on germination medium.  Co-cultivation media contained either 0 or 100 mg/L L-cysteine.  Based on assay at 2 days after inoculation,the transient expression of GUS at scutelar side of explants co-cultivated on medium containing 100 mg/L cysteine was higher than that of the control (0 mg/L cysteine).  Transient expression of GUS on the explants of Tom Thumb was higher than that of Hi-II.  However, transgenic plants in this study were only produced from Hi-II explants co-cultivated in a medium amended with 100 mg/L L-cysteine. No transgenic plants was produced from explants of Tom Thumb due to low efficiency of induction of embriogenic calli.  The efficiency of transformation using explants of Hi-II cocultivated in a medium amended with 100 mg/L L-cysteine was 4 independent transformants or transgenic plants out of 70 explants inoculated or 5.7%. 


Key words:  Agrobacterium tumefaciens, corn, L-cysteine, Hi-II, Tom Thumb

How to Cite
Utomo, S. D. (1). Pengaruh L-Sistein terhadap Efisiensi Transformasi Genetik Jagung (Zea mays) Menggunakan Agrobacterium. Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy), 33(3).