Jurnal Akuakultur Indonesia https://jma.journal.ipb.ac.id/index.php/jai <p>Jurnal Akuakultur Indonesia (JAI) is a scientific journal publishes empirical research and recent science development in aquaculture. JAI is published twice a year in January and July since 2002 by the Indonesian Society of Scientific Aquaculture (ISSA) in association with the Department of Aquaculture, Faculty of Fisheries and Marine Science, Bogor Agricultural University. JAI accepts manuscripts written in English and in Bahasa (Indonesian). JAI only processes submitted original script related to aquaculture and not being published by other publishers. JAI has been registered in some indexing tools like Directory of Open Access Journals (DOAJ), Indonesian BASE, Publication Index (IPI), Google Scholar, Portal Garuda, Mendeley, SINTA, and IPB repository.</p> <p>&nbsp;</p> <p>p-ISSN <a href="http://u.lipi.go.id/1180429182" target="_blank" rel="noopener">1412-5269</a>&nbsp; /&nbsp; e-ISSN <a href="http://u.lipi.go.id/1385091088" target="_blank" rel="noopener">2354-6700</a></p> en-US <p><span>Authors who publish with this journal agree to the following terms:</span></p><br /><ol type="a"><ol type="a"><li>Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a <a href="http://creativecommons.org/licenses/by/3.0/" target="_new">Creative Commons Attribution License</a> that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.</li></ol></ol><br /><ol type="a"><ol type="a"><li>Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.</li></ol></ol><br /><ol type="a"><li>Authors are permitted and encouraged to post their <a title="Click to Continue &gt; by trivia games" href="/index.php/jai/about/submissions#onlineSubmissions">work online<img src="http://cdncache-a.akamaihd.net/items/it/img/arrow-10x10.png" alt="" /></a> (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See <a href="http://opcit.eprints.org/oacitation-biblio.html" target="_new">The Effect of Open Access</a>).</li></ol> akuakultur.indonesia@gmail.com (Prof. Dr. Muhammad Zairin Junior) akuakultur.indonesia@gmail.com (Fitratunisa) Wed, 03 Feb 2021 09:21:29 +0700 OJS 3.1.2.4 http://blogs.law.harvard.edu/tech/rss 60 Evaluation of karamunting Melastoma malabathricum L leaf extract on gonad development and growth performance of tilapia Oreochromis niloticus https://jma.journal.ipb.ac.id/index.php/jai/article/view/32282 <p>The objective of this study was to determine the optimum dose of melastoma <em>M</em><em>elastoma</em><em> malabathricum</em> leaf extract that can inhibit the gonad development of Nile tilapia and increase its growth rate. This study used a completely randomized design containing five extract dose treatments (0, 0.5, 1, 2, and 4 g/kg diet doses) and three replications. The undifferentiated Nile tilapia larvae (7 days post hatching) were randomly distributed (n=30) to fifteen aquaria (100×50×50 cm<sup>3</sup>) and maintained for 112 days using a common recirculation system. The results showed that all dose treatments were not significantly different (P&gt;0.05) in gonadosomatic index values of the D84 and D98 samplings. However, the 1 g/kg diet (D112) was significantly different (P&lt;0.05) in all dose treatments. The final histological results (D112) showed that the 1 g/kg diet obtained the highest inhibition level of the testis and ovary developments, which were still in stage II compared to 0.5 g/kg diet (stage III) and control (stage IV and V). The highest average weight, absolute growth rate, and specific growth rate were obtained in the 1 g/kg diet dose which was significantly different (P&lt;0.05) compared to the control. The percentage of males increased significantly (P&lt;0.05) following the increased dose treatment fed to the fish (4 g/kg diet) with 80.12±4.67%, but the survival rate significantly decreased (P&lt;0.05) compared to the control. The administration of 1 g/kg diet dose obtained the best dose and potential as an inhibiting agent for gonad development in Nile tilapia.</p> <p>&nbsp;</p> <p>Keywords: <em>Melastoma malabathricum</em>, gonad inhibition, cytosterol, <em>Oreochromis niloticus</em></p> <p>&nbsp;</p> <p><strong>ABSTRAK</strong></p> <p><strong>&nbsp;</strong></p> <p>Tujuan dilakukan penelitian ini adalah untuk menentukan dosis optimum ekstrak daun melastoma <em>M</em><em>elastoma</em><em> malabathricum</em> yang dapat menghambat perkembangan gonad ikan nila sehingga meningkatkan laju pertumbuhan somatik dan mengevaluasi efektivitasnya sebagai agen seks reversal alami. Penelitian ini menggunakan rancangan acak lengkap yang terdiri atas lima perlakuan (dosis 0; 0,5; 1,0; 2,0; dan 4,0 g/kg pakan) dan tiga ulangan. Larva ikan nila sebelum kelamin terdiferensiasi (7 hari pascatetas) secara acak (n=30) dimasukkan ke dalam 15 buah akuarium (100×50×50 cm<sup>3</sup>) dan dipelihara selama 112 hari pada sistem resirkulasi. Hasil menunjukkan bahwa semua perlakuan tidak berbeda nyata (P&gt;0,05) terhadap nilai GSI pada sampling D84 dan D98. Namun, perlakuan 1 g/kg pakan pada sampling D112 berbeda nyata (P&lt;0,05) dengan semua perlakuan. Hasil histologi terakhir (D112) menunjukkan bahwa perlakuan 1 g/kg pakan mengindikasikan penghambatan perkembangan testis dan ovari yang paling besar yang masing-masing berada pada TKG II, dibandingkan dengan perlakuan 0,5 g/kg pakan (TKG III), dan dibandingkan dengan kontrol (TKG IV dan TKG V). Pengamatan terhadap bobot rata-rata, laju pertumbuhan mutlak, dan laju pertumbuhan harian tertinggi diperoleh pada perlakuan 1 g/kg pakan yang berbeda nyata (P&lt;0,05) dibandingkan dengan kontrol. Persentase jantan meningkat secara signifikan (P&lt;0,05) seiring meningkatnya konsentrasi ekstrak yang mencapai 80,12±4,67% pada perlakuan 4 g/kg pakan, namun tingkat kelangsungan hidup menurun secara signifikan (P&lt;0,05) dibandingkan dengan kontrol. Pada keseluruhan parameter, pemberian ekstrak 1 g/kg pakan merupakan dosis terbaik dan potensial sebagai agen penghambat perkembangan gonad pada ikan nila.</p> <p>&nbsp;</p> <p>Kata kunci:<em>Melastoma malabathricum</em>, penghambatan gonad, sitosterol, <em>Oreochromis niloticus</em></p> Ermianus Samalei, Muhammad Zairin Jr., Odang Carman, Muhammad Agus Suprayudi Copyright (c) 2021 Jurnal Akuakultur Indonesia https://jma.journal.ipb.ac.id/index.php/jai/article/view/32282 Tue, 02 Feb 2021 11:24:41 +0700 The performance of gold-mouth turban Turbo chrysostomus larvae in different temperature and salinity media https://jma.journal.ipb.ac.id/index.php/jai/article/view/32595 <p>Suhu dan salinitas merupakan parameter kualitas air yang berperan penting terhadap proses fisiologis siput mata bulan (<em>T. chrysostomus</em>) sehingga berdampak terhadap perkembangan, pertumbuhan dan kelangsungan hidup larva. Tujuan dari penelitian ini yaitu untuk mengevaluasi pengaruh suhu dan salinitas terhadap perkembangan, pertumbuhan dan kelangsungan hidup larva siput mata bulan (<em>T</em><em>.</em><em> chrysostomus</em>). Stadia pre-torsion veliger dicapai sekitar 11 jam 36 menit setelah fertilisasi atau sekitar 3 jam setelah trocophor. Stadia post-torsion veliger awal ditandai dengan cangkang yang telah terbentuk sempurna dan pada post-torsion veliger akhir, larva sudah mengembangkan operkulum, kaki, dan propodium. Hasil pengamatan menunjukan bahwa perlakuan A1B3 memberikan waktu pencapaian stadia post-torsion veliger awal dan post-torsion veliger akhir tercepat yaitu masing-masing 19 jam 36 menit dan 22 jam 36 menit setelah pembuahan. Sedangkan perlakuan A1B1 memberikan waktu pencapaian stadia post-torsion veliger awal dan post-torsion veliger akhir terlama yaitu masing-masing 20 jam 30 menit dan 23 jam 25 menit setelah pembuahan. Suhu tidak berpengaruh nyata sedangkan salinitas berpengaruh nyata terhadap laju pertumbuhan harian larva siput mata bulan. Laju pertumbuhan harian tertinggi pada suhu 27±0.5<sup>o</sup>C (A1) tercatat pada perlakuan B3 dan menunjukan nilai yang tidak berbeda nyata dengan perlakuan B2. Suhu dan salinitas memberikan pengaruh yang signifikan namun interaksi keduanya tidak menunjukan pengaruh yang signifikan terhadap tingkat kelangsungan hidup larva siput mata bulan. Perlakuan A1B3 memberikan persentase tingkat kelangsungan hidup tertinggi dan tidak menunjukan nilai yang berbeda nyata dengan perlakuan A1B2. Parameter kualitas air yang diperoleh masih mendukung performa larva siput mata bulan hingga mencapai stadia juvenil.</p> Aris Sando Hamzah, Kukuh Nirmala, Eddy Supriyono, Irzal Effendi Copyright (c) 2021 Jurnal Akuakultur Indonesia https://jma.journal.ipb.ac.id/index.php/jai/article/view/32595 Wed, 10 Feb 2021 09:02:42 +0700 Dietary supplementation of betain to improve the growth and feed utilization in hybrid grouper (Epinephelus lanceolatus♂ × Epinephelus fuscoguttatus♀) juvenile https://jma.journal.ipb.ac.id/index.php/jai/article/view/33775 <p>Betaine plays some important roles in feed utilization and fish metabolism. The aim of this study was to evaluate the effect of dietary betaine supplementation on the growth performance and feed utilizationin hybrid grouper (<em>Epinephelus lanceolatus♂ × Epinephelus fuscoguttatus♀</em><em>)</em>. A completely randomized experimental design with four dietary levels of betaine, i.e.0.0%, 0.5%, 1.0%, and 2.0% in quadruplicate was done.Hybrid grouper juvenile obtained from Brackishwater Aquaculture Development Center, Situbondo, with an initial body length and body weight of 5.89 ± 0.05 cm and 2.86 ± 0.09 g, respectively was used as the tested animal. The fish was maintained in 60 cm x 40 cm x 40 cm aquaria with 75 L working capacity with individual recirculating system with a fish density of 15 fish/aquarium for 50 days. Experimental diet was provided to apparent satiation twice a day. The results of this study demonstrated that dietary betaine at a level of 0.5% resulted in higher feed utilization efficiency, protein and methionine retentions, and growth performance and lower ammonia excretion than those of the control (P&lt;0.05). Higher antioxidative status was indicated by the lower malondialdehyde (MDA) in the liver of fish fed with betaine supplemented diets at levels of 1 - 2%. In conclusion, betaine supplementation of 0.5% could increase feed utilization efficiency and growth performance of hybdrid grouper.</p> <p>&nbsp;</p> <p>Keywods: Betaine, Hybrid Grouper, Growth Performance, Feed, Antioxidative Status</p> <p><strong>ABSTRAK</strong></p> <p>Betain memegang beberapa peranan penting dalam pemanfaatan pakan dan metabolisme pada ikan. Penelitian ini bertujuan untuk mengevaluasi pengaruh suplementasi betain pada pakan terhadap kinerja pertumbuhan dan pemanfaatan pakan pada juvenil ikan kerapu hybrid cantang (<em>Epinephelus lanceolatus♂ × Epinephelus fuscoguttatus♀</em><em>). </em>Penelitian menggunakan rancangan acak lengkap dengan empat perlakuan pakan dengan tingkat suplementasi betain yang berbeda, yaitu 0.0%, 0.5%, 1.0%, dan 2.0% dengan empat ulangan. Juvenil ikan kerapu cantang yang berasal dari Balai Pengembangan Budidaya Laut Situbondo dengan panjang dan bobot awal masing-masing 5.89 ± 0.05 cm and 2.86 ± 0.09 g digunakan sebagai hewan uji dalam penelitian ini. Ikan dipelihara dalam akuarium berukuran 60 cm x 40 cm x 40 cm dengan kapasitas 75L yang dilengkapi dengan sistem resirkulasi individu dengan kepadatan 15 ekor per akuarium selama 50 hari. Pakan uji diberikan hingga sekenyangnya dua kali sehari. Hasil penelitian ini menunjukkan bahwa suplementasi betain sebanyak 0.5% menghasilkan pemanfaatan pakan, retensi protein, retensi metionina, kinerja pertumbuhan dan ekskresi ammonia yang lebih baik daripada kontrol (P&lt;0.05). Status antioksidasi yang lebih juga yang ditunjukkan dengan lebih rendahnya konsentrasi malondialdehid (MDA) pada hati ikan yang diberi pakan dengan suplementasi 1-2% betain. Berdasarkan hasil penelitian dapat disimpulkan bahwa suplementasi betain sebanyak 0.5% dapat meningkatkan efisiensi pemanfaatan pakan dan kinerja pertumbuhan ikan kerapu cantang.</p> Idul La Muhamad, Mia Setiawati, Wiyoto Wiyoto, Julie Ekasari Copyright (c) 2021 Jurnal Akuakultur Indonesia https://jma.journal.ipb.ac.id/index.php/jai/article/view/33775 Mon, 15 Mar 2021 08:57:19 +0700 Growth performance, immune response, and resistance of Nile tilapia fed paraprobiotic Bacillus sp. NP5 against Streptococcus agalactiae infection https://jma.journal.ipb.ac.id/index.php/jai/article/view/32716 <p><strong>ABSTRACT</strong></p> <p><strong>&nbsp;</strong></p> <p>This study was aimed to evaluate the effectiveness of <em>Bacillus</em> sp. NP5 paraprobiotic administration through commercial feed on growth performance, immune response, and resistance of Nile tilapia against <em>Streptococcus agalactiae </em>infection. <em>Bacillus</em> sp. NP5 paraprobiotic was produced through heat-inactivation at 95°C for 1 h, then performed a viability test on <em>tryptic soy agar</em> (TSA) media and incubated for 24 hours. Paraprobiotics could be used whether the bacteria did not grow on the TSA media. This study used a completely randomized design, containing three treatments with five replications, i.e. 1% (v/w) probiotic addition, 1% (v/w) paraprobiotic addition, and no addition of probiotic or paraprobiotic (control). The experimental fish were reared for 30 days. On day 31 of rearing, fish were challenged with <em>S. agalactiae </em>(10<sup>7</sup> CFU/mL) through intraperitoneal injection route, while the negative control was injected with PBS. This study results significantly improved growth performances and immune responses (P&lt;0.05), compared to control after 30 days of probiotic and paraprobiotic <em>Bacillus</em> sp. NP5 administration. After challenge test, increased immune responses in probiotic and paraprobiotic of <em>Bacillus</em> sp. NP5 treatment had higher survival rates (P&lt;0.05) than positive control. The administration of <em>Bacillus</em> sp. NP5 probiotic and paraprobiotic through commercial feed were effective in increasing growth performance, immune response, and resistance of Nile tilapia against <em>S. agalactiae</em> infection.</p> <p>Keywords: <em>Bacillus</em> sp. NP5, Nile tilapia, paraprobiotic, <em>Streptococcus agalactiae</em></p> <p><strong>&nbsp;</strong></p> <p><strong>ABSTRAK</strong></p> <p><strong>&nbsp;</strong></p> <p>Penelitian ini bertujuan mengevaluasi efektivitas pemberian paraprobiotik <em>Bacillus</em> sp. NP5 melalui pakan dalam meningkatkan kinerja pertumbuhan, respons imun, dan resistansi ikan nila terhadap infeksi <em>Streptococcus agalactiae</em>. Proses pembuatan bakteri paraprobiotik yaitu <em>Bacillus</em> sp. NP5 diinaktivasi panas pada suhu 95°C selama 1 jam, dilanjutkan dengan pengujian viabilitas dengan menyebarkannya pada media <em>tryptic soy agar </em>kemudian diinkubasi selama 24 jam. Jika bakteri tidak tumbuh, maka paraprobiotik dapat digunakan. Penelitian ini menggunakan rancangan acak lengkap dengan tiga perlakuan dan lima ulangan, yaitu penambahan probiotik 1% (v/w), penambahan paraprobiotik 1% (v/w), dan tanpa penambahan probiotik atau paraprobiotik (kontrol). Ikan perlakuan dipelihara selama 30 hari dan pada hari ke-31, ikan diuji tantang dengan <em>S. agalactiae</em> (10<sup>7</sup> CFU/mL) melalui injeksi intraperitoneal, sementara perlakuan kontrol negatif diinjeksi dengan PBS. Hasil penelitian setelah 30 hari pemberian probiotik dan paraprobiotik <em>Bacillus </em>sp. NP5 menunjukkan kinerja pertumbuhan dan respons imun yang meningkat signifikan (P&lt;0.05) dibandingkan dengan kontrol. Pascauji tantang, peningkatan respons imun pada perlakuan probiotik dan paraprobiotik <em>Bacillus </em>sp. NP5 menunjukkan tingkat kelangsungan hidup yang lebih tinggi (P&lt;0.05) dibandingkan kontrol positif. Pemberian probiotik dan paraprobiotik <em>Bacillus</em> sp. NP5 melalui pakan dapat meningkatkan kinerja pertumbuhan, respons imun, dan resistansi ikan nila terhadap infeksi <em>Streptococcus</em> <em>agalactiae.</em></p> <p>Kata kunci: <em>Bacillus</em> sp. NP5, ikan nila, paraprobiotik, <em>Streptococcus</em> <em>agalactiae</em></p> Aldy Mulyadin, Widanarni Widanarni, Munti Yuhana, Dinamella Wahjuningrum Copyright (c) 2021 Jurnal Akuakultur Indonesia https://jma.journal.ipb.ac.id/index.php/jai/article/view/32716 Tue, 16 Mar 2021 09:18:33 +0700 Effects of nano-scale nutrients supplement on natural productivity of Thalassiosira sp. and growth performance of Pacific white shrimp, Litopenaeus vannamei, reared under intensive conditions using concrete tank culture system https://jma.journal.ipb.ac.id/index.php/jai/article/view/34028 <p>ABSTRACT</p> <p>The aim of this study was to evaluate the use of unique mixture of nano-nutrient to extent the growth of diatom Thalassiosira sp. and the effect to the water quality, growth performance, and protein composition on the whole body of the Pacific white shrimp Litopenaeus vannamei. There are four treatments with four replicates per treatment with the use of commercial nano-nutrients (Aquaritin Aquaculture or AA) namely: (1) 0.70 mg/L; (2) 0.525 mg/L, (3) 0.35 mg/L, and (4) without any AA application, but included standard application of using urea and NPK fertilizers to enhance the growth of diatom. The use of AA was successful to trigger the growth of Thalassiosira sp. Group of shrimp treated with 0.70 mg/L had better growth rate. Results of feeding trial indicated that adding AA could also improve the biomass, final mean weight, survival, percentage weight gain, and better feed utilization in terms of FCR. The addition of AA to enhance the growth of Thalassiosira sp. also provides a beneficial impact to the protein composition in whole body of shrimp. Biologically, the protein composition in the whole body of shrimp treated with 0.7 mg/L was higher. The findings from this study showed that the addition of commercial nano-nutrient could enhance the growth of Thalassiosira sp. and led to better growth of shrimp cultured in concrete tank</p> <p>Key words: Growth, Litopenaeus vannamei, nano-nutrients, protein composition, Thalassiosira sp.</p> <p>ABSTRAK</p> <p>Tujuan penelitian yaitu mengevalusi penggunaan campuran unik nano-nutrien untuk meningkatkan pertumbuhan diatom Thalassiosira sp. dan pengaruhnya terhadap kualitas air, laju pertumbuhan, dan komposisi protein pada tubuh udang L. vannamei. Terdapat empat perlakuan dan empat ulangan per perlakuan dengan penggunaan nano-nutrisi komersial (Aquaritin Aquaculture atau AA) yaitu: (1) 0,70 mg/L; (2) 0,525 mg/L; (3) 0,35 mg/L, dan (4) tanpa pemberian AA, tetapi menggunakan penerapan standar pupuk urea dan NPK untuk pertumbuhan diatom. Penggunaan AA berhasil memicu pertumbuhan Thalassiosira sp. Kelompok udang yang diberi perlakuan 0,70 mg/L memiliki laju pertumbuhan yang lebih baik. Hasil uji coba pemberian pakan menunjukkan bahwa penambahan AA juga dapat meningkatkan biomassa, bobot rata-rata akhir, kelangsungan hidup, persentase pertambahan bobot dan pemanfaatan pakan yang lebih baik dalam hal FCR. Penambahan AA untuk meningkatkan pertumbuhan Thalassiosira sp. juga memberikan dampak yang menguntungkan bagi komposisi protein di seluruh tubuh udang. Secara biologis komposisi protein di tubuh udang dengan perlakuan 0,70 mg/L lebih tinggi. Hasil dari kajian ini menunjukkan bahwa penambahan nano-nutrien komersial dapat meningkatkan pertumbuhan Thalassiosira sp. dan mendorong pertumbuhan udang bada sistem budidaya di bak beton.</p> <p>Kata kunci: Komposisi protein, Litopenaeus vannamei, nano-nutrien, pertumbuhan, Thalassiosira sp.</p> Romi Novriadi, Rifqi Fadhilah, Aldy Eka Wahyudi, Dea Ananda Prayogi, Ilham Ilham, Sunil Nanda Copyright (c) 2021 Jurnal Akuakultur Indonesia https://jma.journal.ipb.ac.id/index.php/jai/article/view/34028 Tue, 23 Mar 2021 10:14:59 +0700