Performance Comparison of Single and Two-Phase Biogas Digesters Treating Dairy Cattle Manure at Tropical Ambient Temperature

  • S. Sutaryo Department of Animal Sceince, Faculty of Animal and Agricultural Sciences, Diponegoro University
  • A. N. Sempana Department of Animal Science, Faculty of Animal and Agricultural Sciences, Diponegoro University
  • C. M. S. Lestari Department of Animal Science, Faculty of Animal and Agricultural Sciences, Diponegoro University
  • A. J. Ward Department of Engineering, Faculty of Science and Technology, Aarhus University
Keywords: biogas, manure, tropical ambient temperature, two-phase digester biogas


The biodegradation process of organic waste in anaerobic digestion can be in a single or two-phase bio-reactor. This study examined the effect of different biogas digester configurations (single and two-phase) on methane production of dairy cattle manure (DCM) at tropical ambient temperature. Three identical reactors were used in this study (R1, R2, and R3). The two-phase digesters consisted of reactors R1 and R2. R1 had a 2.1 L working volume and 3 d hydraulic retention time (HRT), while R2 had 5.25 L working volume and 22 d HRT (R1 and R2 had a 25 d HRT). The digested slurry of R1 was used to feed R2. R3 served as the single-phase digester and had 5.25 L working volume and 25 d HRT. Methane production were 14.31, 132.82, and 146 L/kg VS for R1, R2, and R3, respectively. The results showed that there was no positive effect of the application of a two-phase digester configuration on the specific methane yield of DCM per kg volatile solids added than that in the single-reactor. Methane production was detected in the first reactor of the two-phase digester configuration and the total methane production of the two-phase digester was found to be 29.98% higher (p<0.05) than that of the single reactor in terms of digester volume (0.41 VS 0.31 L/L/d). Both digester configurations performed well, indicated by a stable methane production and low volatile fatty acids and total ammonia concentrations. The two-phase bio-digester configuration can significantly increase methane production in terms of digester volume.


Download data is not yet available.


Adekunle, K. F. & J. A. Okolie. 2015. A review of biochemical process of anaerobic digestion. Adv. Biosci. Biotechnol. 6:205-212.

APHA. 1995. Standard Methods for Examination of Water and Waste Water. 19th Ed. American Public Health Association, Washington DC.

Bandara, W. M. K. R. T. W., T. Kindaichi, H. Satoh, M. Sasakawa, Y. Nakahara, M. Takahashi, & S. Okabe. 2012. Anaerobic treatment of municipal wastewater at ambient temperature: Analysis of archaeal community structure and recovery of dissolved methane. Water Res. 46: 5756-5764.

Baldi, F., L. Pecorini, & R. Iannelli. 2019. Comparison of single-stage and two-stage anaerobic co-digestion of food waste and activated sludge for hydrogen and methane production. Renew Energy. 43:1755-1765.

Beneragama, N., S. A. Lateef, M. Iwasaki, T. Yamashiro, & K. Umetsu. 2013. The combined effect of cefazolin and oxytetracycline on biogas production from thermophilic anaerobic digestion of dairy manure. Bioresour. Biotechnol. 133:23-30.

Burton, C.H. & C. Turner. 2003. Anaerobic treatment options for animal manures. In: Manure Management - Treatment Strategies for Sustainable Agriculture, 2nd Ed, chapter 7. Silsoe Research Institute, as part of the EU Accompanying Measure project, MATRESA, Wrest Park, Silsoe, Bedford, UK.

Brown, D. & Y. Li. 2013. Solid state anaerobic co-digestion of yard waste and food waste for biogas production. Bioresour. Technol. 127: 275-280.

Chae, K. J., S. K. Am Jang, L. Yim, & S. Kim. 2008. The effects of digestion temperature and temperature shock on the biogas yields from the mesophilic anaerobic digestion of swine manure. Bioresour. Technol. 99: 1-6.

Ganesh, R., M. Torrijos, P. Sousbie, A. Lugardon, J. P. Steyer, & J. P. Delg. 2014. Single-phase and two-phase anaerobic digestion of fruit and vegetable waste: Comparison of start-up, reactor stability and process performance. Waste Manag. 34: 875-885.

Gomez, K. A. & A. A. Gomez. 2007. Prosedur Statistik untuk Penelitian Pertanian. Translated by Sjamsuddin, E., & J. S. Baharsjah. UI Press, Jakarta.

Linke, B., A. Rodríguez-Abalde, C. Jost, & A. Krieg. 2015. Performance of a novel two-phase continuously fed leach bed reactor for demand-based biogas production from maize silage. Bioresour. Technol. 177: 34-40.

Mao, C., Y. Feng, X. Wang, & G. Ren. 2015. Review on research achievements of biogas from anaerobic digestion. Renew. Sust. Energ. Rev. 45: 540-555.

Minale, M. & T. Worku. 2014. Anaerobic co-digestion of sanitary wastewater and kitchen solid waste for biogas and fertilizer production under ambient temperature: waste generated from condominium house. Int. J. Environ. Sci. Technol. 11: 509-516.

Murugan, N. & P. Appavu. 2018. Investigation on low temperature biogas generation. Int. J. ambient energy. 39:1-3.

Noorollahi, Y., M. Kheirrouz, H. F. Asl, H. Yousefi, & A. Hajinezhad. 2015. Biogas production potential from livestock manure in Iran. Renew. Sust. Energ. Rev. 50: 748-754.

Rao, P. V., S. S. Baral, R. Dey, & S. Mutnuri. 2010. Biogas generation potential by anaerobic digestion for sustainable energy development in India. Renew. Sust. Energ. Rev. 14: 2086-2094.

Sinbuathong, N., P. Sirirote, B. Sillapacharoenkul, J. Munakata-Marr, & S. Chulalaksananukul. 2012. Biogas production from two-stage anaerobic digestion of jatropa curcas seed cake. Energ. Source Part A. 34: 2048-2056.

Sutaryo, S., A. J. Ward, & H. B. Møller. 2012. Thermophilic anaerobic co-digestion of separated solids from acidified dairy cow manure. Bioresour. Technol. 114: 195-200.

Sutaryo, S., A. J. Ward, & H. B. Møller. 2014. The effect of mixed-enzyme addition in anaerobic digestion on methane yield of dairy cattle manure. Environ. Technol. 35: 2476-2482.

Tsigkou, K., P. Tsafrakidou, A. Kopsahelis, D. Zagklis, C. Zafiri, & M. Kornaros. 2020. Used disposable nappies and expired food products valorization through one- & two-stage anaerobic co-digestion. Renew. Energy. 147: 610-619.

Wei, S., H. Zhang, Z. Cai, J. Xu, J. Fang, & H. Liu. 2014. Psychrophilic anaerobic co-digestion of highland barley straw with two animal manures at high altitude for enhancing biogas production. Energy convers. manag. 88: 40-48.

Yenigün, O. & B. Demirel. 2013. Ammonia inhibition in anaerobic digestion: A review. Process Biochem. 48: 901-911.

How to Cite
Sutaryo, S., Sempana, A. N., Lestari, C. M. S., & Ward, A. J. (2020). Performance Comparison of Single and Two-Phase Biogas Digesters Treating Dairy Cattle Manure at Tropical Ambient Temperature. Tropical Animal Science Journal, 43(4), 354-359.