Species Authentication of Dog, Cat, and Tiger Using Cytochrome β Gene

  • . Irine
  • H Nuraini
  • C Sumantri
Keywords: cat, cytochrome β gene, dog, multiplex PCR, tiger

Abstract

Adulteration of animal food products for economic reason has happened during the last decades. Species identification method development was needed to prevent falsification information. The objective of this research was to study species authentication (dog, cat, and tiger) to ensure animal origin in products using cyt β gene specific marker. DNA extraction and fragment amplification were conducted using phenol-chloroform and multiplex PCR (Polymerase Chain Reaction) method, respectively. This research showed that fragment length of amplification for species tested (dog, cat, and tiger) were 523, 331, 319 bp, respectively. Species specificity was also indicated by high reverse primers homology percentage. Multiplex PCR technique succeed to amplify DNA fragment from species tested, but has a limitation to amplify total DNA composite of mix DNA.

Author Biographies

. Irine

Major Program of Animal Science Production and Technology, Graduate School, Bogor Agricultural University

H Nuraini
Department of Animal Production and Technology, Faculty of Animal Science, Bogor Agricultural University
C Sumantri
Department of Animal Production and Technology, Faculty of Animal Science, Bogor Agricultural University
Published
2014-03-10