Genetic Variation of mtDNA Cytochrome Oxidase Subunit I (COI) in Local Swamp Buffaloes in Indonesia

  • F Saputra
  • . Jakaria
  • C Sumantri
Keywords: COI, filogeny, genetic variation, Indonesian buffaloes

Abstract

The objective of this research was to identify genetic variation of mitochondria DNA especially in cytochrome oxidase subunit I (COI) among population of Indonesian buffaloes. Samples of swamp buffaloes were collected from Aceh (n= 3), North Sumatra (n= 3), Riau (n= 3), Banten (n= 3), Central Java (n= 3), West Nusa Tenggara (n= 3) and South Sulawesi (n= 3), and riverine buffalo from North Sumatra (n= 1) out of group for comparison. Sequence of COI was analyzed using MEGA 5.10 software with neighbor-joining method kimura 2-parameter model to reconstruct phylogeny tree. The result showed that three haplotypes for swamp buffalo and one haplotype for riverine buffalo in Indonesia resulted from 41 polymorphic sites. This finding showed that the COI gene could be considered as a marker to distinguish among swamp buffaloes in Indonesia.

Author Biographies

F Saputra

Major Program of Animal Science Production and Technology, Graduate School, Bogor Agricultural University

. Jakaria

Department of Animal Production and Technology, Faculty of Animal Science, Bogor Agricultural University
Jln. Agatis, Kampus IPB Darmaga, Bogor 16680, Indonesia

C Sumantri

Department of Animal Production and Technology, Faculty of Animal Science, Bogor Agricultural University
Jln. Agatis, Kampus IPB Darmaga, Bogor 16680, Indonesia

Published
2014-03-10