The Incorporation of Chromium in Rice Straw Fermented with Ganoderma lucidum

  • F Agustin
  • T Toharmat
  • D Evvyernie
  • D Taniwiryono
  • S Tarigan
Keywords: chromium, Ganoderma lucidum, incorporation, rice straw

Abstract

The research was designed to evaluate chromium incorporation by Ganoderma lucidum in rice straw substrate supplemented with chromium chloride. Treatments were combination of Cr level (0, 500, 1000, 1500, 2000, 25000, and 3000 ppm) and fermentation time (0, 2, 3, and 4 wk). The treatments were arranged in a factorial 7 x 4 and allocated in completely randomized design with three replications.  G. lucidum was grown in potato dextrose agar (PDA) medium for 10 days and was inoculated to the substrate which have been sterilized and mixed with CrCl3.6H2O. The moisture of substrate was maintained at 70%. Fiber and protein components of growth media of G. lucidum was determined and analyzed for their Cr content. The finding result showed that addition of Cr up to 3000 ppm into the substrate stimulated the G. lucidum growth.  Chromium was incorporated into the fiber and protein components of the growth substrate of G. lucidum during fermentation. Incorporation of Cr into the protein of substrate containing3000 ppm Cr  was highest when fermented for 4 wk.  Protein component of substrate contained 9.29% Cr while in NDF and ADF was 27.20% and 10.55% Cr, respectively. It is concluded that Cr was incorporated into the G. lucidum cells during fermentation. 

Author Biographies

F Agustin

Department of Nutrition and Feed Technology, Graduate Program, Bogor Agricultural University

Faculty of Animal Science, Andalas University
Kampus Limau Manis Padang, Indonesia 

T Toharmat
Department of Nutrition and Feed Technology, Faculty of Animal Science, Bogor Agricultural University
D Evvyernie
Department of Nutrition and Feed Technology, Faculty of Animal Science, Bogor Agricultural University
D Taniwiryono

Indonesian Biotechnology Research Institute for Estate Crops
Jln. Salak No. 1, Bogor, Indonesia

S Tarigan

Research Institute of Veterinary Science
Jln. R.E. Martadinata 30, PO Box 151, Bogor, 16114, Indonesia

Published
2013-05-29