Improvement on the Nutritive Quality of Napier Grass Silage through Inoculation of Lactobacillus plantarum and Formic Acid

  • Saprilian Stya Hapsari Study Program of Nutrition and Feed Science, Faculty of Animal Science, Graduate School, Bogor Agricultural University
  • Suryahadi Suryahadi Department of Nutrition and Feed Technology, Faculty of Animal Science, Bogor Agricultural University
  • Heri Ahmad Sukria Department of Nutrition and Feed Technology, Faculty of Animal Science, Bogor Agricultural University
Keywords: napier grass silage, fermentation additive, Lactobacillus plantarum, formic acid

Abstract

The potential availability of forage feed is high, but in reality this potential has not been able to meet the requirement of feed both in sustainable quantity and quality. Silage made with the use of liquid fermentation additive (FA) can be a solution for those problems. The use of  different levels of FA and addition of Lactobacillus plantarum bacteria as well as formic acid were expected to improve the nutritive quality of napier grass silage. The first experiment was designed to measure the fermentative quality of napier grass silage. The treatments used were the levels of FA, L. plantarum, and formic acid supplementations. The experiment used a completely randomized design with a 3x2x2 factorial arrangement with 3 replications. The first factor was the level of liquid FA (5%, 7.5%, and 10%), and the second factor was the inoculation of L. plantarum (without and with inoculation of the L. plantarum), and the third factor was the addition of formic acid (without and with the addition of 0.15% formic acid). The second experiment was aimed to evaluate chemical and microbiological characteristics, and in vitro digestibility of selected napier grass silage. The results showed that napier grass silage from all treatments showed good qualities. There were interactions between FA, L. plantarum, and formic acid on DM content (P<0.05) and ammonia production (P<0.01). The use of FA showed an interaction (P<0.01) with the addition of L. plantarum and formic acid in Fleigh point. Ammonia production in rumen (P<0.01), total VFA (P<0.05), and in vitro digestibility (P<0.01) were significantly affected by the treatments. The optimal level of liquid FA was 7.5%. Based on the nutritive quality of silage, L. plantarum addition was as effective as control treatment to improve nutritive quality of napier grass silage through the increased  of fermentation characteristics i.e., low pH, high DM product, high fermentation product (VFA), and digestible on rumen. Formic acid reduced ammonia production during ensiling and fermentation in rumen, but it was less effective in inhibiting the fermentation process when it was combined with L. plantarum.

References

Abarghoei, M., Y. Rouzbehan, & D. Alipour. 2011. Nutritive value and silage characteristics of whole and partly stoned olive cakes treated with molasses. J Agr Sci Tech. 13:709-716.

Adesoji, A. T., A. A. Ogunjabi, O. E. Fagade & O. J. Babayemi. 2010. Effect of Lactobacillus plantarum starter culture on the microbial succession, chemical composition, aerobic stability and acceptability by ruminant of fermented Panicum maximum grass. AU J. T. 14:11-24.

Adesoji, A. T., A. A. Ogunjobi, O. J. Babayemi, & S. Omotosho. 2012. Physicochemical screening of Lactobacillus plantarum and its effects on fermentation of Panicum maximum grass for silage production. World J. Agric Sci. 8:547-559.

Aksu, T., E. Baytok, M. A. Karsh, & H. Muruz. 2006. Effect of formic acid, molasses and inoculant additives on corn silage composition, organic matter digestibility and microbial protein synthesis in sheep. Small Rum. Rec. 61:29-33. http://dx.doi.org/10.1016/j.smallrumres.2004.12.013

Arasu, M. V., M. W. Jung, D. H. Kim, S. Ilavenil, M. Jane, H. S. Park, N. A. Al-Dhabi, B. T. Jeon, & K. C. Choi. 2014. Enhancing nutritional quality of silage by fermentation with Lactobacillus plantarum. Indian J. Micr. Indian J. Micr. 54: 396-402.

AOAC. 2005. Official Methods of Analysis of AOAC International. 18th ed. Assoc. of Anal. Chem., Arlington.

Baker, S. B. & W. H. Summerson. 1941. The colorimetric determination of lactic acid in biological material. J. Biol. Chem. 138:535-554.

Baumont, R., S. Prache, M. Meuret, & P. Morand-Fehr. 2000. How forage characteristics influence behavior and intake in small ruminants: a review. Livestock Prod. Sci. 64:15-28. http://dx.doi.org/10.1016/S0301-6226(00)00172-X

Bureenok S., T. Namihira, S. Mizumachi, Y. Kawamoto, & T. Nakada. 2006. The effect of epiphytic lactic acid bacteria with or without different byproduct from defatted rice bran and freen tea waste on napier grass (Pennisetum purpureum Shumach) silage fermentation. J. Sci. Food Agric. 86:1073-1077. http://dx.doi.org/10.1002/jsfa.2458

Boucher, S.E, R. S. Ordway, N. L. Whitehouse, F. P. Lundry, P. J. Kononoff, & C. G. Schwab. 2007. Effect of incremental urea supplementation of a conventional corn silage-based diet on ruminal ammonia concentration and synthesis of microbial protein. J. Dairy Sci. 90:5619-5633. http://dx.doi.org/10.3168/jds.2007-0012

Cappuccino, J.G. & N. Sherman. 2001. Microbiology; a Laboratory Manual. 6th ed. State University of New York, New York.

CENTRAS (Center for Tropical Animal Studies). 2013. Produksi Hijauan Fermentasi (Hi-fer+) dalam Kemasan Komersial Menggunakan Probiotik Unggul untuk Pencapaian swasembada Daging. Laporan Akhir Pengembangan Ilmu Pengetahuan dan Teknologi (IPTEK). CENTRAS, Bogor.

De Boever, J. L., E. Dupon, E. Wambacq & J. Latre. 2013. The effect of a mixture of Lactobacillus strains on silage quality and nutritive value of grass harvested at four growth stages and ensiled for two periods. Agric. Feed Sci. 22:115-126.

De Oliveira, S. G., T. T. Berchielli, R. A. Reis, M. E. Vechetini, & M. S. Pedreira. 2009. Fermentative characteristics and aerobic stability of sorghum silages containing different tannin levels. Anim. Feed Sci. and Tech. 154:1-8. http://dx.doi.org/10.1016/j.anifeedsci.2009.07.003

Ferreira, G. & D. R. Merten. 2005. Chemical and physical characteristics of corn silages and their effects on in vitro disappearance. J. Dairy Sci. 88:4414-4425. http://dx.doi.org/10.3168/jds.S0022-0302(05)73128-3

Filya, I. 2003. The effect of Lactobacillus buchneri and Lactobacillus plantarum on the fermentation, aerobic stability, and ruminal degradability of low dry matter corm and sorghum silages. J. Dairy Sci. 86:3565-3581. http://dx.doi.org/10.3168/jds.S0022-0302(03)73963-0

Filya, I. & E. Sucu. 2007. The effect of bacterial inoculants and a chemical preservation on the fermentation and aerobic stability of whole-crop cereal silage. Asian-Aust. J. Anim. Sci. 20(3):378-384.

General Laboratory Procedures. 1966. Department of Dairy Science. University of Wisconsin, Madison.

Guo, X. S., W. R. Ding, J. G. Han, & H. Zhou. 2008. Characterization of protein fractions and amino acids in ensiled alfalfa treated with different chemical additives. Anim Feed Sci and Tech. 142:89-98. http://dx.doi.org/10.1016/j.anifeedsci.2007.07.005

Idikut, L., B. A. Arikan, M. Kaplan, I. Guven, A. I. Atalay, & A. Kamalak. 2009. Potential nutritive value of sweet corn as a silage crop with or without corn ear. J of Anim and Vet Adv. 8:734-741.

Jaakkola, S., V. Kaunisto, & P. Huhtanen. 2006. Volatile fatty acid proportions and microbial protein synthesis in the rumen of cattle receiving grass silage ensiled with different rates of formic acid. Grass and For. Sci. 61:282-292. http://dx.doi.org/10.1111/j.1365-2494.2006.00532.x

Kennedy, S. J. 1990. Comparison of the fermentation quality and nutritive value of sulfuric and formic acid-treated silages fed to beef cattle. Grass For Sci. 47:75-87.

KiliÇ, A. 1986. Silo Feed. Bilgehan Publ, Izmir.

Lazzarini, I., E. Detmann, C. B. Sampaio, M. F. Paulino, S. C. V. Filho, M. A. de Souza, & F. A. Oliveira. 2009. Intake and degistibility in the cattle fed low-quality tropical and supplemented with nitrogenous compounds. R. Bras. Zootec. 38(10):2021-2030. http://dx.doi.org/10.1590/S1516-35982009001000024

Lee, M. R. F., M. B. Scott, J. K. S. Tweed, F. R. Minchin, & D. R. Davies. 2008. Effects of polyphenol oxidase on lipolysis and proteolysis of red clover silage with and without a silage inoculants (Lactobacillus plantarum L54). J Anim Feed Sci and Tech. 144:125-136. http://dx.doi.org/10.1016/j.anifeedsci.2007.09.035

Li, Y. & N. Nishino. 2011. Bacterial and fungal communities of wilted Italian ryegrass silage inoculated with and without Lactobacillus rhamnosus or Lactobacillus buchneri. Lett. Appl. Microbiol. 52:314-321. http://dx.doi.org/10.1111/j.1472-765X.2010.03000.x

Liu, Q., C. Wang, C. X. Pei, H. Y. Li, Y. X. Wang, S. L. Zhang, Y. L. Zhang. J. P. He, H. Wang, W. Z. Yang, Y. S. Bai, Z. G. Shi, & X. N. Liu. 2014. Effect of isovalerate supplementation on microbial status and rumen enzyme profile in steers fed on corn stover based diet. Livestock Sci. 60-68. http://dx.doi.org/10.1016/j.livsci.2013.12.034

Lorenzo, B. F. & P. O’Kiely. 2008. Alternatives to formic acid as a grass silage additive under two contrasting ensilability conditions. Irish J. of Agric and Food Resc. 47:135-149.

Luckstadt, C. 2009. Acidifiers in Animal Nutrition: A Guide for Feed and Acidification to Promote Animal Performance. Nottingham Univ. Press, UK.

McDonald, P., A. R. Henderson, & S. J. E. Heron. 1991. The Biochemistry of Silage. 2th ed. Chalcombe Publications, Marlow, UK.

Pathak, A. K. 2008. Various factors affecting microbial protein synthesis in the rumen. Vet. World. 1(6): 186-189.

Ratnakomala, S., R. Ridwan, G. Kartika, & Y. Widyastuti. 2006. Pengaruh inokulum Lactobacillus plantarum 1A-2 dan 1BL-2 terhadap kualitas silase rumput gajah (Pennisetum purpureum). Biodiversitas. 7(2):131-134. http://dx.doi.org/10.13057/biodiv/d070208

Rowghani, E. & M. J. Zamiri. 2009. The effects of a microbial inoculants and formic acid as silage additives on chemical composition, ruminal degradability and nutrient digestibility of corn silage in sheep. Iranian J. of Vet Resc, Shiraz Univ. 10(2):110-118.

Saarisalo, E., T. Jalava, E. Skytt, A. Haikara, & S. Jaakola. 2006. Effect of lactid acid bacteria inoculant, formic acid, potassium sorbate and sodium benzoate on fermentation quality and aerobic stability of wilted grass silage. Agric. Food Sci. 15: 185-199. http://dx.doi.org/10.2137/145960606779216263

Saarisalo, E., E. Skytt, A. Haikara, T. Jalava, & S. Jaakkola. 2007. Screening and selection of lactic acid bacetria strains suitable for ensiling grass. J. of Appl. Micr. 102:327-336.

Santoso, B., B. Tj. Hariadi, H. Manik, & H. Abubakar. 2011. Silage quality of king grass (Pennisetum purpureoidhes) treated with epiphytic and commercial LAB. Med. Pet. 34:140-145. http://dx.doi.org/10.5398/medpet.2011.34.2.140

Santoso, B., B. Tj. Hariadi, V. Sabariah, & T. Sraun. 2014. Fermentation quality and in vitro nutrient digestibility of fresh rice straw-based silage treated with lactic acid bacteria. Med. Pet. 37:115-120. http://dx.doi.org/10.5398/medpet.2014.37.2.115

Steel, R.G. D. & J, H, Torrie. 1997. Principles and Procedures of Stastistics. McGraw-Hill, New York (US).

Tilley, J. M. A & R. A. Terry. 1963. A two-stage technique for the in vitro digestion of forage crops. J. British Grasslan Soc. 18: 104-111. http://dx.doi.org/10.1111/j.1365-2494.1963.tb00335.x

Van Soest, P. J., J. B. Robertson, & B. A. Lewis. 1991. Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition. J. Dairy Sci. 74:3583-3597. http://dx.doi.org/10.3168/jds.S0022-0302(91)78551-2

Widyastuti, Y. 2008. Fermentasi silase dan manfaat probiotik silase bagi ruminansia. Med. Pet. 31:225-232.

Published
2016-08-29