Extraction of Bioactive Compounds as Natural Antioxidants from Fresh Spirulina platensis using Different Solvents

  • Fiya Firdiyani Program Studi Teknologi Hasil Perikanan Jurusan Perikanan, Fakultas Perikanan dan Ilmu Kelautan, Universitas Diponegoro, Jalan Prof. Soedarto SH, Tembalang, Semarang 50275. Telepon/faks: (024)7474698
  • Tri Winarni Agustini Program Studi Teknologi Hasil Perikanan Jurusan Perikanan, Fakultas Perikanan dan Ilmu Kelautan, Universitas Diponegoro, Jalan Prof. Soedarto SH, Tembalang, Semarang 50275. Telepon/faks: (024)7474698
  • Widodo Farid Ma'ruf Program Studi Teknologi Hasil Perikanan Jurusan Perikanan, Fakultas Perikanan dan Ilmu Kelautan, Universitas Diponegoro, Jalan Prof. Soedarto SH, Tembalang, Semarang 50275. Telepon/faks: (024)7474698

Abstract

Spirulina platensis is a bioactive-rich microalga of great potential as a source of natural antioxidant. Natural antioxidants are increasing in demand, because it is considered as more safe than synthetic antioxidants. The objective of this study was to determine the effect of solvent on the potential of natural antioxidant from fresh S. platensis. The antioxidant compound was extracted using either acetone or ethyl acetate by reflux method. The yield of extractions were 1.86% and 3.07% for aceton and ethyl acetate, respectively. Fresh S. platensis extracts were considered as potential antioxidant source with IC50 values 65.89 ppm, and 76.36 ppm when using acetone and ethyl acetate, respectivetly, even though it were still lower compare to the IC50 of positive control quercetin i.e 21.64 ppm. Phytochemical test showed positive results of phenolic compound, triterpenoids, steroid, flavanoid, and saponin. aw value of Spirulina platensis was 0.61 and 0.81 for aceton and ethyl acetate, respectively. It was concluded that different solvent affected the antioxidant activity of S. platensis extract. Keywords: Antioxidants, aceton, ethyl acetate, fresh Spirulina platensis
Published
2015-06-25