DNA Mini-Barcodes as A Molecular Marker for Various Hairtail Fish Products Traceability

  • Asadatun Abdullah Departemen Teknologi Hasil Perairan, Fakultas Perikanan dan Ilmu Kelautan, Institut Pertanian Bogor Jalan Lingkar Akademik Kampus IPB Dramaga, Bogor, Jawa Barat
  • Mala Nurilmala Departemen Teknologi Hasil Perairan, Fakultas Perikanan dan Ilmu Kelautan, Institut Pertanian Bogor Jalan Lingkar Akademik Kampus IPB Dramaga, Bogor, Jawa Barat
  • Kinanti Permata Sitaresmi Departemen Teknologi Hasil Perairan, Fakultas Perikanan dan Ilmu Kelautan, Institut Pertanian Bogor Jalan Lingkar Akademik Kampus IPB Dramaga, Bogor, Jawa Barat
Keywords: authentication, primer design, DNA Mini-barcodes, hairtail fish, mislabelling

Abstract

Hairtail fishery is an important fishery commodity with high economic value as export commodity. Authentication based on DNA mini-barcodes can be applied to overcome seafood fraud and to support Indonesian traceability system. This study was aimed to design species-specific primers of DNA minibarcodes and authentication of various processed hairtail fish products such as smoked, baked, fried, canned, and boiled. Analytical method applied PCR-based of cytochrome c oxidase 1 (COI) full length and
mini-barcodes. Mini-barcode primer specific for hairtail (Trichiurus lepturus) successfully amplified DNA of processed hairtail fish. The BLAST analysis showed the samples were identified as Trichiurus sp. and T. lepturus with homology level of 91% to 100%. Phylogenetic analysis showed the processed sample (LC dan LD) were in the same group with T. lepturus.
Published
2019-04-30