Sperm quality of Litopenaeus vannamei broostock injected by PMSG and antidopamin

  • Fahmi Akbar Departemen Budidaya Perairan Institut Pertanian Bogor
  • Agus Oman Sudrajat Departemen Budidaya Perairan Institut Pertanian Bogor
  • Siti Subaidah Departemen Budidaya Perairan Institut Pertanian Bogor

Abstract

ABSTRACT

 

The important role in determining the productivity of shrimp was the quality and quantity of shrimp sperm. The decreasing of hatching rate was predicted as the effect of the decreasing quality of sperm. It then could  influence  the number and quality of naupli produced. Hormonal induction of maturation is one of alternative solution that can improve shrimp sperm quality. This study was conducted to examine the effect of pregnant mare serum gonadotropin (PMSG) and antidopamine (AD) injection on white shrimp Litopenaeus vannamei sperm quality. This research consisted of six treatments which were treatment without eyestalk ablation, eyestalk ablation, and premix PMSG hormone, and AD at the dose  of 0.1 mL/kg, 0.25 mL/kg, 0.5 mL/kg, and 1 mL/kg. The observed parameters were sperm count and percentage of normal and abnormal sperm. The results showed that PMSG hormone and AD injection could improve sperm quality of L. vannamei shrimp. Hormone at the dose of 0.25 mL/kg and 0.5 mL/kg were the optimal doses to increase sperm count and the percentage of normal sperm, also to lower the percentage of abnormal sperm.

 

Keyword: PMSG, AD, sperm quality, Litopenaeus vannamei

 

 

ABSTRAK

 

Kuantitas dan kualitas sperma udang jantan sangat berperan penting dalam menentukan produktivitas udang. Terjadinya penurunan daya tetas telur udang diduga karena terjadinya penurunan kualitas sperma. Hal ini berpengaruh terhadap jumlah dan kualitas nauplius yang diproduksi. Induksi maturasi secara hormonal merupakan salah satu alternatif yang dapat meningkatkan kualitas sperma udang. Penelitian ini dilakukan untuk mengkaji pengaruh penyuntikan pregnant mare serum gonadotropin (PMSG) dan antidopamin (AD) terhadap kualitas sperma udang vaname Litopenaeus vannamei. Penelitian terdiri atas enam perlakuan, yaitu perlakuan tanpa ablasi mata, ablasi mata, dan injeksi dengan premix hormon PMSG dan AD dosis 0,1 mL/kg, 0,25 mL/kg, 0,5 mL/kg, dan 1 mL/kg. Parameter yang diamati jumlah sperma, persentase sperma normal dan abnormal. Hasil penelitian menunjukkan penyuntikan hormon PMSG dan AD dapat meningkatkan kualitas sperma udang L. vannamei. Hormon dosis 0,25 mL/kg dan 0,5 mL/kg merupakan dosis optimal dalam meningkatkan jumlah sperma dan persentase sperma normal, serta mengurangi persentase sperma abnormal.

 

Kata kunci: PMSG, AD, kualitas sperma, Litopenaeus vannamei

 

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Published
2015-10-15
How to Cite
Akbar, F., Sudrajat, A. O., & Subaidah, S. (2015). Sperm quality of Litopenaeus vannamei broostock injected by PMSG and antidopamin. Jurnal Akuakultur Indonesia, 14(2), 98-103. https://doi.org/10.19027/jai.14.98-103