Skip to main content Skip to main navigation menu Skip to site footer

Identifikasi Potensi Enzim Lipase dan Selulase pada Sampah Kulit Buah Hasil Fermentasi

  • La Ode Sumarlin
  • Dikdik Mulyadi
  • . Suryatna
  • Yoga Asmara
Keywords: cellulase, DNS method, fermentation, fruit peel waste, lipase


Fermentation is one of bioconversion to produce profitable anaerobic microbes and to produce various enzymes. Lipases and cellulases are widely used enzymes so far. Cellulases play an important role in bioconversion of organic waste cellulosic materials to glucose, single cell proteins, animal feed, and ethanol. Lipases can also degrade fatty ester bond. Therefore, both enzymes are potential to be used in industry as well as in households. Fermentation of fruit peel waste is an attempt to produce cellulase and lipase that can be carried out in a simple way. Cellulase as says was performed using DNS (3.5-dinitrosalicylic acid) and acid-base titration for analysis of lipase using cooking oil as the substrate. The results showed that the highest cellulase activity was obtained from watermelon rind mixed with citrus fruit peel of 0.036 U/mL, and mixed of banana peel and citrus fruit, which was 0.035 U/mL. The optimum lipase activity was at 30 oC, pH 7, and reaction time of 60 minutes. The highest lipase activity (1.36 U/mL) was obtained from mixture of watermelon and orange rind. Thus, the fruit peel waste is potential to produce cellulase and lipase by fermentation .


Download data is not yet available.

Author Biographies

La Ode Sumarlin
Fakultas Sains dan Teknologi, Universitas Islam Negeri Syarif Hidayatullah Jakarta, Ciputat 15412
Dikdik Mulyadi
Fakultas Sains dan Teknologi, Universitas Muhammadiyah Sukabumi, Sukabumi 43113
. Suryatna
Fakultas Sains dan Teknologi, Universitas Muhammadiyah Sukabumi, Sukabumi 43113
Yoga Asmara
Fakultas Sains dan Teknologi, Universitas Muhammadiyah Sukabumi, Sukabumi 43113


Al-Sayed HMA, Ahmed AR. 2013. Utilization of watermelon rinds and sharlyn melon peels as a natural source of dietary fiber and antioxidants in cake. Annals of Agric. Sci.. 58(1): 83–95.

Alvarez R, Liaden G. 2007. Semicontinuous co-digestion of solid slaughterhouse waste, manure, and fruit and vegetable waste. J Renew Energy. 33: 726-734.

Asih S. 2011. Karakterisasi enzim hidrolase bakteri dari mata air soda Parbubu, Tapanuli Utara. [Skripsi]. Bogor (ID): Institut Pertanian Bogor.

Bisswanger H. 2013. Review: Enzyme assays. Perspectives in Science. 1:41–55.

Bussamara R, Fuentefria MA, Oliveira ES, Broetto L, Simcikova M, Valente P, Schrank A, Vainstein MH. 2008. Isolation of lipase secretion yeast for enzyme production in a pilot-plant scale batch fermentation. Biores Technol. 101: 268-275.

Chanda S, Bravalia Y, Kaneria M, Rakholiya K, 2010. Fruit and Vegetable peels- strong natural source of antimicrobics. Curent Research, Technology and Education Topic in Appl Microbiol Microb Biotechnol.: 444-450.

Daniel RM, Danson MJ. 2013. Review: Temperature and the catalytic activity of enzymes: A fresh understanding. FEBS Letters. 587: 2738–2743.

Delgado CIH, Fleuri LH. 2014. Short communication: Obtaining lipases from by products of orange juice processing. Food Chem. 163: 103–107.

Dewi ERS. 2013. Kadar Lipase dan Protease pada Fermentasi Kombucha dengn Variasi Teh (Camelia sinensis). Bioma. 2(1): 64-73.

Feng X, Patterson DA, Balaban M, Emanuelsson EAC. 2013. Characterization of tributyrin hydrolysis by immobilized lipase on woollen cloth using conventional batch and novelspinning cloth disc reactor. Chem. Eng. Res. Des. 91: 1684–1692.

Ferrer M, Soliveri J, Plou FJ, Cortes LN, Duarte RD, Christensen M, Patino CJL, Ballesteros A. 2005. Synthesisof sugar esters in solvent mixtures by lipases from Thermomyces lanuginosus and Candida antarctica B, and their antimicrobial properties. Enzyme Microb. Technol. 36: 391–398.

Gulati R, Isar J, Kumar V, Prasad AK, Parmar VS, Saxena RK. 2005. Production of a novel alkaline lipase by Fusarium globulosum using neem oil, and itsapplications. Pure Appl. Chem. 77(1): 251–262.

Ganjar I, Sjamsurdzal W, Oetari A. 2006. Mikologi dasar dan terapan yayasan Obor Indonesia. Jakarta (ID).

Gunam IBW, Aryanta WR, Darma IBNS. 2011. Produksi selulase kasar dari kapang Trichoderma viride dengan perlakuan konsentrasi substrat ampas tebu dan lama fermentasi. J Biol. 15: 29-33.

Gupta R, Gupta N, Rathi P. 2004. Bacterial lipases: an overviewof production, purification and cbiochemical properties. Appl.Microbiol. Biotechnol. 64: 763–781.

Irawan B, Sutihat, Sumardi. 2008. Uji aktivitas enzim selulase dan lipase pada mikrofungi selama proses dekomposisi limbah cair kelapa sawit dengan pengujian kultur murni. Bandarlampung (ID): Universitas Lampung. 284-291.

Jin Z, Liang S, Zhang X, Han S, Ren C, Lin Y, dan Zheng S. 2013. Synthesis of fructose laurate esters catalyzed by aCALB-displaying Pichia pastoris whole-cell biocatalyst in anon-aqueous system. Biotechnol. Bioprocess Eng. 18: 365–374.

Kim DW, Jeong YK, Jang YH, Lee JK. 1984. Purification and characteristic of endoglucanase and exoglucanase component from Trichoderma viride. J Ferment Bioeng. 77(4): 363-369.

Kamini NR, Iefuji H. 2001. Lipase catalyzed methanolysis of vegetable oils in aqueous medium by Cryptococcus spp. S-2. Process Biochem. 37: 405–410.

Kartal F, Kilinc A, Timur S. 2007. Lipase biosensor fortributyrin and pesticide detection. Int. J. Environ. Anal. Chem. 87: 715–722.

Krogh KBRM, Kastberg H, Jørgensen CI, Berlin A, Harris PV, Olsson L. 2009. Cloning of a GH5 endoglucanase from genus Penicillium and its binding to different lignins. Enzyme and Microbial Technology. 44: 359-367.

Miller GL. 1959. Use of Dinitrosalicylic Acid Reagent of Determination of Reducing sugar. Anal Chem. 31: 246-248.

Murni SW, Kholisoh DS, Tanti DI, Petrisia EM. 2011. Produksi, karakterisasi, dan isolasi lipase dari Aspergillus niger. Pengembangan Teknologi Kimia untuk Pengolahan Sumberdaya Alam Indonesia. ISSN 1693-4393.

Pasaribu FL, Yenie E, Muria SR. 2010. Pengaruh konsentrasi Substrat dan Waktu Fermentasi Pada Pemanfaatan Limbah Kulit Nenas (Ananas Comosus L.Merr). Pekan Baru (ID): Universitas Riau.

Rahman M, Sen PK, Hasan FM, Miah, Rahman HM. 2004. Purification and Characterization of Invertase Enzyme from Sugarcane. J Biol Sci, 7(3): 340-345.

Rajan A, Nair J. 2011. A comparative study on alkaline lipase production by a newly isolated Aspergillus fumigatus MTCC 9657 in submerged and solid-state fermentation using economically and industrially feasible substrate. Turk J Biol. 35: 569-574.

Ribeiro BD, de Castro Am, Coelho MA, Freire DM. 2011 Production an use of lipases in bioenergy: A Review: The Feedstock to Biodiesel Production. University of Rio de Janeiro (BR). Brazil.

Rodibillah S, Muharam S, Panji T. 2011. Produksi Diasilgliserol (DAG) sebagai Minyak Sehat dan Emulsifier dan Emulsifier dari Crude Palm Oil (CPO). [Skripsi]. Sukabumi (ID): Universitas Muhammadiyah Sukabumi.

Rosyana E. 1995. Pemanfaatan Kulit Pisang Untuk Produksi Enzim Selulase. [Skripsi]. Bogor (ID): Institut Pertanian Bogor.

Sa’adah Z, Noviana IS, Abdullah. 2010. Produksi Enzim Selulase Oleh Asperillus niger Menggunakan Substrat Jerami dengan Sistem Frementasi Padat. Semarang (ID): Universitas Diponegoro.

Sadhu S, Maiti TK. 2013. Cellulase Production by Bacteria: A Review. British Microb. Research J. 3(3): 235-258.

Schlegel HG. 1994. Mikrobiologi umum. Ed. Keenam. Terjemahan: Baskoro, RMT dan Wattimena JR. Gadjah Mada University Press, Yogyakarta (ID).

Singhania RR, Sukumaran RK, Patel AK, Larroche C, Pandey A. 2010. Advancement and comparative profiles in the production technologies using solid-state and submerged fermentation for microbial cellulases. Enzyme Microb. Technol. 46: 541–549.

Subowo YB. 2010. Uji Aktivitas Enzim Selulase dan Ligninase Dari Beberapa Jamur dan Potensinya Sebagai Pendukung pertumbuhan Tanaman Terong (Solanum Melongena). Bogor (ID): Lembaga Ilmu Pengetahuan Indonesia.

Tamires CS, Gomes DPP, Bonomo RCF, Franco M. 2011. Optimization of Solid State Fermentation of Potato Peel for The Production of Cellulolytic Enzymes. Food Chem. 133: 1299-1304.

Thirunavukarasu K, Edwinoliver NG, Anbarasan SD, Gowthaman MK, Iefuji H, Kamini NR. 2008. Removal oftriglyceride soil from fabrics by a novel lipase from Cryptococcus sp. S-2. Process Biochem. 43: 701–706.

Underwood AL, Raday JR. 2001. Analisis Kimia Kuantitatif ed. 6 penerjemah: Iis Sopyan, Jakarta (ID): Erlangga. Terjemahan dari Quantitative Analysis: 141.

Winterhalter C, Liebl W. 1995. Two extremely thermostable xylanase of the hyper-thermophilic bacterium Thermotoga maritima MSBB Appl Environ Microbiol. 61(5):1810-1815.

Yau EW, Rosnah S, Noraziah M, Chin NL, Osman H. 2010. Physico-chemical compositions of the red seedless watermelons (Citrullus Lanatus) International Food Research J. 17: 327-334.

Yuzo K, Sakaya S. 2003. Purification and characterization of the lipase from Pseudomonas fluorescens HU 380. J. of biosci. and bioengin. 96(3): 211-226.

How to Cite
Sumarlin, L. O., Mulyadi, D., Suryatna, ., & Asmara, Y. (2014). Identifikasi Potensi Enzim Lipase dan Selulase pada Sampah Kulit Buah Hasil Fermentasi. Jurnal Ilmu Pertanian Indonesia, 18(3), 159-166. Retrieved from http://jma.journal.ipb.ac.id/index.php/JIPI/article/view/8394