Variasi Genetik Gen Myostatin Ekson 3 pada Sembilan Bangsa Kambing Lokal di Indonesia
Myostatin gene plays a role in helping to control the growth and development of muscle tissue. Identification of genetic diversity in nine local goat breeds in Indonesia has done. The aim of this study was to obtain information myostatin gene diversity of exon 3 in local goats in Indonesia. The total of 10 samples was selected from 80 samples of goat's blood collected comprising each sample of the population of goats breeds, i.e., Samosir, Muara, Kacang, Costa, Peranakan Etawah, Boerawa, Gembrong, Boer, and Boerka goats. The gene diversity and nucleotide base changes were identified by using polymerase chain reaction (PCR) and sequencing techniques. The analysis showed that there is eight variant identified in appropriate with those found in sequencing results. Deletion variations were found in Costa and Samosir goats in T552- and G560-. Substitution variations were found in Gembrong (A7C & A11T), Peranakan Etawah (T10A & A11T), Burawa (T10A & A11T), Muara (A11T), Samosir (A11T), and Boerka (A182T, T437A, T439A, & A445G). Variations on the chromatogram peak overlapping contained in the base position to 13. Analysis of variance showed that there was a special mutation in exon 3 that affects the amino acid tyrosine into lysine. Variants were found in nine goat breeds associated with phylogenetic and genetic distance of goats, Boerka goat has the highest level of genetic variation, it indicated that Boerka goat was crossbreed
Alakilli SYM, Mahrous KF, Salem LM, Ahmed ES. 2012. Genetic polymorphisme of five genes associated with growth traits in goat. African Journal of Biotechnology. 11(82): 14738-14748.
An XP, Wang JG, Hou JX, Zhao HB, Bai L, Li G, Wang LX, Liu XQ, Xiao WP, Song YX, Cao BY. 2011. Polymorpism identification in the goat MSTN gene and association analysis with growth traits. Czech Journal of Animal Science. 56(12): 529-535.
Batubara A. 2011. Studi Keragaman Fenotipik dan Genetik Beberapa Sub Populasi Kambing Lokal Indonesia dan Strategi Pemanfaatannya Secara Berkelanjutan. [Disertasi]. Bogor (ID): Institut Pertanian Bogor.
Boman IA, Klemetsdal G, Blichfeldt T, Nafstad O, Vage DI. 2009. A frameshift mutation in the coding region of the myostatin gene (MSTN) affects carcass conformation and fatness in norwegian white sheep (Ovis aries). Animal Genetics. 40(4): 418-422. http://doi.org/fm4pjt
Byun SO, Fang Q, Zhou H, Hickford JGH. 2009. An effective method for silver - staining DNA in large numbers of polyacrylamide gels. Analytical Biochemistry. 385(1): 174-175. http://doi.org/fpbz6z
Ferguson A. 1980. Biochemical Systematics and Evolution Lecturer in Zoology. [Undergraduate Thesis]. London (GB): The Queens University of Belfast.
Gan SQ, Du Z, Liu SR, Yang YL, Shen M, Wang XH, Yin JL, Hu XX, Fei J, Fan JJ, Wang JH, He QH, Zhang YS, Li N. 2008. Association of SNP haplotypes at the myostatin gene with muscular hypertrophy in sheep. Asian-Australasian Journal of Animal Sciences. 21(7): 928-935. http://doi.org/b898
Hall T. 2004. BioEdit version 7.0.0. Isis Pharmaceuticals, Inc.
Kostaman T, Sutama IK. 2005. Laju pertumbuhan kambing anak hasil persilangan antara kambing boer dengan peranakan etawah pada periode pra-sapih. Jurnal Ilmu Ternak dan Veterniner. 10(2): 106-112.
Li XL, Wu ZhL, Gong YF, Liu YQ, Liu ZZ, Wang XJ, Xin TR, Ji Q. 2006a. Single nucleotide polymorphism identification in the caprine myostatin gene. Journal of Animal Breeding and Genetics. 123: 141-144. http://doi.org/cdg58w
Li XL, Wu ZL, Liu ZZ, Gong YF, Zhou RY, Zheng GR. 2006b. SNP identification and analysis in part of intron 2 of goat MSTN gene and variation within and among species. Journal of Heredity. 97(3): 285-289. http://doi.org/dqbzc9
Li XL, Liu ZZ, Zhou RY, Zheng GR, Gong YF, Li LH. 2008. Deletion of TTTTA in 5’UTR of goat MSTN gene and its distribution in different population groups and genetic effect on bodyweight at different ages. Frontiers of Agriculture in China. 2(1): 103-109. http://doi.org/bvh5xn
Mahmilia F, Tarigan A. 2006. Karakteristik morfologi dan performans kambing kacang, kambing boer dan hasil persilangannya. Lokakarya Nasional Kambing Potong. 1(2): 209-2012.
Noor RR. 2008. Genetika Ternak. Jakarta (ID): Penebar Swadaya.
Rusdiana S, Prahararli L, Adiati U. 2014. Prospek dan strategi perdagangan ternak kambing dalam merebut peluang pasar dunia. Agriekonomika. 3(2): 204-223.
Sambrook JEF, Fritsch EF, Maniatis T. 1989. Molecular Cloning a Laboratory Manual. 2nd Ed. Cold Spring Harbor Laboratory Pr.
Sodiq A, Adjisoedarmo S, Tawfik ES. 2003. Reproduction rate of kacang and peranakan etawah goats under village production system in Indonesia. Internasional Research on Food Security, Natural Resource Management and Rural Development. 8(10): 1-7.
Sulabda IN, Susari NW, Heryani LGS, Puja K. 2012. Genetic diversity of gembrong goat based on DNA microsatellite markers. Global Veterinaria. 9(1): 113-116.
Tamura K, Nei M, Kumar S. 2004. Prospects for inferring very large phylogenies by using the neighbor-joining method. Proceedings of the National Academy of Sciences of the United States of America (PNAS). 101(30): 11030-11035. http://doi.org/fth7tb
Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. 2013. MEGA 6: Molecular evolutionary genetics analysis version 6.0. Molecular Biology and Evolution. 30(12): 2725-2729. http://doi.org/5v5
Zhang ZJ, Ling YH, Wang LJ, Hang YF, Guo XF, Zhang YH, Ding JP, Zhang XR. 2013. Polymorphisms of the myostatin gene (MSTN) and its relationship with growth traits in goat breeds. Genetics and Molecular Research. 12(2): 965-971. http://doi.org/f4t3vs
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